<x-flowed>Michael,
my most recent experience with adjacent phosphorylation sites is described
in:
Mitchelhill KI, Michell BJ, House CM, Stapleton D, Dyck J, Gamble J, Ullrich
C, Witters LA, Kemp BE.
Posttranslational modifications of the 5'-AMP-activated protein kinase beta1
subunit.
J Biol Chem. 1997 Sep 26;272(39):24475-9.
this protein is phosphorylated on either of two adjacent Serines (Ser24/25)
as well as on other sites. In every condition of in-vitro phosphorylation I
tried, I could never get two phosphates in, it was always either one or the
other with a preference for Ser24. I always figured steric hindrance was at
work. If you find a protein that fits your requirements, I would very much
like to hear.
Regards....Ken
>From: Michael Curtis <curtism@mail.upstate.edu>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: Neighboring Phosphorylation Sites
>Date: Mon, 11 Jun 2001 16:23:49 -0400
>
>Hello.
>
>I am currently working on a phosphorylation project and have a question
>concerning neighboring phosphorylation sites. Does anyone know of an
>example of
>two phosphorylateable residues being directly next to each other and both
>being
>phosphorylated? Or sites that are very close?
>
>For example:
>
>Directly next to each other:
>
>i.e XXSTXX giving XXSpTpXX upon phosphorylation
>
>Or very close by:
>
>i.e. XXS(X1-2)TXX giving XXSp(X1-2)TpXX upon phosphorylation
>
>I am wondering if phosphorylation of one would sterically hinder the
>phosphorylation of the other. Any instance of these cases that you know
>about or
>any insight you have on this issue would be greatly appreciated.
>
>Thanks in advance.
>
>Mike
>
>Mike Curtis
>UMU
>curtism@mail.upstate.edu
>
>
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