Thank you very much, Sasha. That site is exactly what I needed,
especially since this is a protein from gram+ bacteria.
Scottie
. At 9:25 AM +0200 6/12/01, Alexandre Podtelejnikov wrote:
>Dear Scottie,
>
>You can also try to predict signal peptide using the followeing link
>http://www.cbs.dtu.dk/services/.
>It bssed on neural networks,
>
>y64rs,
>Sasha
>
>-----Original Message-----
>From: Association of Biomolecular Resource Facilities
>[mailto:abrf-request@aecom.yu.edu]On Behalf Of Scottie Adams
>Sent: 11. juni 2001 21:12
>To: Recipients of ABRF List
>Subject: protein Expression
>
>
>Hi
>
> I have a secreted protein that I need to express. I would like to
>remove the native leader sequence and substitute the appropriate leader
>sequence for the expression system that I will be using. However, the
>leader sequence for the protein that I wish to express is not known for
>sure.
>
>I'm looking for a website that will allow me to paste in my protein
>sequence and it will predict what the leader sequence is based on
>hydrophobicity, etc.
>Thanks
>
>Scottie
>
>Scottie Adams aka Pamela Scott Adams
>
>Manager
>Molecular Biology Core Facility
>Trudeau Institute
>100 Algonquin Avenue
>Saranac Lake, NY 12983
>Phone: 518-891-3080, Ext. 115
>Fax: 518-891-5126
>Email: sadams@northnet.org
>sadams@trudeauinstitute.org
>http://www.trudeauinstitute.org
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