At 04:23 PM 6/11/2001 -0400, Michael Curtis wrote:
>Hello.
>
>I am currently working on a phosphorylation project and have a question
>concerning neighboring phosphorylation sites. Does anyone know of an
example of
>two phosphorylateable residues being directly next to each other and both
being
>phosphorylated? Or sites that are very close?
>
>For example:
>
>Directly next to each other:
>
>i.e XXSTXX giving XXSpTpXX upon phosphorylation
>
>Or very close by:
>
>i.e. XXS(X1-2)TXX giving XXSp(X1-2)TpXX upon phosphorylation
>
>I am wondering if phosphorylation of one would sterically hinder the
>phosphorylation of the other. Any instance of these cases that you know
about or
>any insight you have on this issue would be greatly appreciated.
>
>Thanks in advance.
>
>Mike
>
>Mike Curtis
>UMU
>curtism@mail.upstate.edu
>
>
>
>
>
Mike,
Cardiac Troponin I has two adjacent Ser residues (Human, Bovine, etc)
that can be phosphorylated in vitro AND in vivo. Recombinant Human form
available from Spectral Diagnostics in Canada.
Regards,
Dan L. Crimmins, Ph.D.
Washington University School of Medicine
Department of Immunology and Pathology
Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone 314-454-8514
Fax 314-454-5208
e-mail crimmins@pathbox.wustl.edu
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