Re: protein Expression

From: Thomas Weber (Thomas.Weber@mssm.edu)
Date: Tue Jun 12 2001 - 12:14:32 EDT


Hi,
try this link:

http://www.cbs.dtu.dk/services/SignalP/

hope this helps

Thomas

>Hi
>
> I have a secreted protein that I need to express. I would like to
>remove the native leader sequence and substitute the appropriate leader
>sequence for the expression system that I will be using. However, the
>leader sequence for the protein that I wish to express is not known for
>sure.
>
>I'm looking for a website that will allow me to paste in my protein
>sequence and it will predict what the leader sequence is based on
>hydrophobicity, etc.
>Thanks
>
>Scottie
>
>Scottie Adams aka Pamela Scott Adams
>
>Manager
>Molecular Biology Core Facility
>Trudeau Institute
>100 Algonquin Avenue
>Saranac Lake, NY 12983
>Phone: 518-891-3080, Ext. 115
>Fax: 518-891-5126
>Email: sadams@northnet.org
>sadams@trudeauinstitute.org
>http://www.trudeauinstitute.org

-- 
Dr. Thomas Weber
Institute for Gene Therapy and Molecular Medicine
Box 1496
Mount Sinai School of Medicine
1425 Madison Avenue
New York, NY 10029-6574
United States of America

Phone (office): (212) 659 8293 Phone (lab): (212) 659 8299 Fax: (212) 849 2437 e-mail: thomas.weber@mssm.edu http://www.mssm.edu/genetherapy/weber/



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