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Subject: Re: disulphide bond prediction
Date: Wed, 13 Jun 2001 15:43:32 +0900
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Hi Gene,
Hello from Yokohama in JAPAN.
It looks late reply, and may be LITTLE informative for you.
I found
PROTEIN SEQUENCING PROTOCOLS Edited by Bryan John Smith
Methods in Molecular Biology Volume.64
ISBN 0-89603-353-8
In Chapter30,
Quintification and Location of Disulfide Bonds in Proteins
About LC-MS info. is NOT there.But Method for HPLC are there.
I also have strong interests to use your developing system.Regards,
Takeshi Fukuhara
tfukuhar@bio.titech.ac.jp
http://takeiteasy.4mg.com/
http://www1.bio.titech.ac.jp/~tkishimo/ref/ronbun-e.html
----- Original Message -----
From: "Gene Wijffels" <Gene.Wijffels@li.csiro.au>
To: "Recipients of ABRF List" <abrf@aecom.yu.edu>
Sent: Thursday, May 24, 2001 7:10 PM
Subject: disulphide bond prediction
> Hi ABRFers,
> I am trying to use protein digests and LC-MS to determine the disulphide
> bond arrangements within a couple of smallish knotty proteins.
>
> Does someone know of a web site that possesses a program that can
> predict/calculate/indicate the probable disulphide bonds of a (obviously
> non-reduced) protein given the mass of peptides from a digest, the
> protein's primary sequence, the endoproteinase specificity (and the buffer
> conditions of the digest if relevant)?
>
> I am aware of sites and programs that predict the mass of fragments and
> products of partial digests, but I need something smarter.
>
> Thanks heaps in advance,
> Gene
> Gene Wijffels
> CSIRO Livestock Industries
>
> Phone: (07) 3346 2510/14
> FAX: (07) 3346 2509
>
> Please note new postal address:
>
> CSIRO Livestock Industries,
> Level 3 Gehrmann Laboratories,
> Research Road
> University of Queensland
> St Lucia Qld 4072
>
>
> street address: Level 7
> Gehrmann Laboratories
> Research Road
> University of Queensland 4072
>
>
>
>
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