Date: Wed, 01 Jan 1997 15:49:08 +0800
From: "Dr. Ming F. Tam" <MBR405@ccvax.sinica.edu.tw>
Subject: ProtSeq:Bulk filter prep
To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Does anyone have a good method for prepping glass fiber filters for
>sequencing peptides? It takes so much seq time to prep them.
>Lisa
Aebersold published a paper in 1987 [PNAS 84, 6970] for prepping glass
fiber filters for sequencing. It works well with proteins. We have not
tried this method with peptide samples. Instead, we apply the peptide
samples directly onto a small piece of Problot membranes under light
suction from a water pump. It works great and it is cheap.
Dr. Ming F. Tam
Institute Molecular Biology
Academia Sinica
mbr405@ccvax.sinica.edu.tw