Date: Thu, 02 Jan 97 10:03:47 -0500
From: "Thomas J Miller" <millertj@a1.esvax.umc.dupont.com>
Message-Id: <11800120107991/4868375@ESVAX>
Subject: RE: spotting samples on ProBlot
To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Dr. Ming F. Tam wrote:
Aebersold published a paper in 1987 [PNAS 84, 6970] for prepping glass
fiber filters for sequencing. It works well with proteins. We have not
tried this method with peptide samples. Instead, we apply the peptide
samples directly onto a small piece of Problot membranes under light
suction from a water pump. It works great and it is cheap.
==================================
Are there any reasons one could not use a heat lamp to dry the sample on
Problot (or other pvdf membrane products)? Thank you. Tom
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Thomas Miller, Macromolecular Analysis, Protein Sequencing
Dupont Co., POB 80328, Wilmington, DE 19880
Voice 302-695-1745
email: millertj@esvax.dnet.dupont.com
Opinions are my own and not of my employer.
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=