Date: Tue, 7 Jan 1997 11:12:43 -0800 (PST)
From: "PPMAL (Hathaway/Krapf)" <ppmal@cco.caltech.edu>
To: "ABRF Hypermail (Dirk Krapf)" <abrfhyp@cco.caltech.edu>
Subject: Resolution in Chromatography (fwd)
Date: Tue, 07 Jan 1997 14:48:47 +0100
From: Peter Hunziker <phunzi@bioc.unizh.ch>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Subject: Resolution in Chromatography
I have a theoretical question for all the chromatography specialists among
the list members.
During the preparation of a lecture on chromatography I have found two
different expressions for the calculation of the resolution:
1. R = 0.25 (alpha-1) (square root of N) (k/k+1)
2. R = 0.25 (alpha-1/alpha) (square root of N) (k/k+1)
In both cases the definition of alpha is the same (k2/k1). Can anybody
explain me if and how these two equations are correlated to each other?
Which is the "right" one?
Many thanks and bets regards
Peter
**************************************************************
Dr. Peter Hunziker
Universitaet Zuerich
Biochemisches Institut
Winterthurerstr 190
CH-8057 Zuerich
Switzerland
Tel: +41-1-257 55 20
Fax: +41-1-363 79 47
E-Mail: phunzi@bioc.unizh.ch