Date: Tue, 07 Jan 1997 13:51:52 -0500 (EST)
From: Mark Lively <lively@mgrp.bgsm.edu>
Subject: PepSyn
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Two questions for the peptide chemists in the audience.
1. We wish to make a short peptide (7 residues) that contains an
N-terminal (3-thio)propionic acid residue. It will look like an
N-terminal Cys without its amino group. Using FastMoc chemistry on an ABI
430 synthesizer, I propose to add the thiopropionyl "residue" as its
4-methyl-benzyl-3-thio propionic acid derivative coupled using a normal
amino acid coupling cycle. Assuming this coupling should work, will the
4-methyl-benzyl protecting group be removed by TFA using typical FMOC
deprotection cocktails? This group has been used in t-Boc chemistry to
make peptides containing this group and is apparently readily removed
using HF. Alternatively, we could potentially use Trityl-thio-propionate,
the analog of Trityl-Cys that we currently use for our Cys derivatives
but this would require obtaining Trityl-3-thio-propionic acid.
2. Does anyone know of a source for Fmoc-homoarginine(Pmc)-OH?
(Pmc = pentamethylchroman-6-sulphonyl)
Many thanks in advance and New Year's greetings to all, especially the
"small angel" at the helm of this cyber ship.
\ml
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Mark O. Lively, Ph.D. Voice: 910-716-2969
Professor of Biochemistry Fax: 910-716-7671
Bowman Gray School of Medicine email: Lively@mgrp.bgsm.edu
Wake Forest University
Winston-Salem, NC 27157
Home page: http://www.bgsm.edu/molecular_genetics/
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