Message-Id: <v03007813aef8b405702a@[129.186.10.114]>
Date: Tue, 7 Jan 1997 20:19:39 -0600
From: "Harold G. Hills" <hhills@iastate.edu>
Subject: DNA Seq. Long Ranger
To: Recipients of ABRF List <abrf@aecom.yu.edu>
I am making my second attempt at using LongRanger at the 4X speed on the
377. I would like to know the recipe that is being used. LR, TBE, urea,
water, Temed and APS. Are you degassing and if so for how long. My first
attempt using the FMC recipe was poor beyong 450 bases compared to my
cheap, cheap 29:1 acrylamide.
Hal
Harold G. Hills, Ph.D. DNA Sequencing Specialist 515 294-9585
1184 Molecular Biology Building FAX 515 294-1597
Iowa State University
Ames, IA 50011-3260 hhills@iastate.edu