Date: Wed, 8 Jan 1997 11:00:40 -0600
Message-Id: <v01530503aefa80db6d9e@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: Re: D-His and Other Problems
Janice,
To resolve your issue of racemization, we have followed the procedure
by Adamson, Lajoie and others, Analytical Biochemistry 202, 210-214,
(1992). Their procedure is very thorough and reproducible. We have
performed the experiment by hydrolyzing a control peptide (which you
say you've synthesized) along with the suspect. This way you can
measure the amount of racemization which occurs in hydrolysis and
derivatization naturally. The amino acids are then analyzed on a C18
reversed phase column.
Also, this was the topic of this year's ABRF study. Perhaps some listener
out there (who is not at the EPS) knows what the procedure was to
determine extent of racemization.
Best regards,
Cynthia G. Fields
University of Minnesota
Dept. Lab. Med. Path.
Biomedical Engineering Center
voice: (612) 626-1900 fax: 624-7967
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110