From: "Fowler, Audree" <AFOWLER@biochem.medsch.ucla.edu>
Subject: FW: Tutorial Information for ABRF '97
Date: Wed, 08 Jan 97 13:10:00 PST
To: Recipients of ABRF List <abrf@aecom.yu.edu>
You may have noticed in the December newsletter that there are now 7
Tutorials on Sunday, February 9 in Baltimore. all of the Tutorials are
listed here.
You can either register for the Tutorials on the FASEB web site or
simply Email your name and the Tutorial you want to attend to:
kmirabal@faseb.org
FAX and phone are listed at end of this message.
We may try to get some of these tutorials on the web so again we are
asking for notetakers to help out. Of course you will get in if you take
notes.
We still need a couple of people for "Peptides from Hell", and one more
for DNA sequence analysis.
Please send your interest to:
Audree Fowler
afowler@biochem.medsch.ucla.edu
FAX: 310 206-5272
ABRF '97 TUTORIAL SESSIONS
Seven Tutorials have been scheduled for Sunday, February 9 beginning at
12:00 Noon. Those interested in participating must register by using the
registration form. Advance registration is strongly encouraged as courses
are
limited to 80 participants. On-site registration will only be allowed on
a
space available basis. The cost of the tutorial is included in the
meeting
registration fee.
12:00 PM - 2:00 PM
CONCURRENT TUTORIAL SESSIONS
TUTORIAL: ACCESSING THE GENOME DATABASE
CONSTELLATION BALLROOM F
Chair: David J. Lipman, NLM, NIH
TUTORIAL: MASS SPECTROMETRY
CONSTELLATION BALLROOM E
Chair: Kristine M. Swiderek, Beckman Res. Inst.
This workshop will introduce you to the most actual mass spectral
techniques
for the analysis of proteins and peptides. The tutorial will cover topics
which are of relevance for the use of mass spectrometry in Core
Laboratories.
It will include tandem mass spectrometry, interpretation of MS/MS
spectra,
microcapillary LC-MS, microflow techniques, ion-trap mass spectrometry,
MALDI-TOF, delayed extraction (DE) and post source decay (PSD). Most of
these
techniques will be presented in the context of the structu
ral analysis of proteins and peptides. In addition, some mass spectral
techniques for the analysis of non-protein and non-peptide material will
be
discussed.
John J. Lennon, Univ. of Washington
Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass
Spectrometry
Murray Hackett, Univ. of Washington
Practical Aspects of Performing MS/MS Experiments using the Triple
Quadrupole
Mass Spectrometer
Michael T. Davis, Beckman Res. Inst.
Variable-Flow Microcapillary LC System Design for the Analysis of Complex
Peptide Mixtures via On-line LC/MS Interfaces
TUTORIAL: OPERATION OF CORE LABS
CONSTELLATION BALLROOM D
Chair: Ronald L. Niece, Univ. of California, Irvine
Ronald L. Niece, Univ. of California, Irvine
The Business of Running a Core Facility: What it Takes to be
Business-Like
Duane A. Bartley, Johns Hopkins Univ.
The Business of Running a Core Facility: Handling High Volume Throughput
Issues
Lisa Bibbs, Scripps Res. Inst.
The Business of Running a Core Facility: Personnel Issues and Management
Training
TUTORIAL: GLYCOPROTEINS
CONSTELLATION BALLROOM C
Chair: Daniel R. Marshak, Osiris Therapeutics, Inc.
Identification and analysis of glycosyl residues in glycopeptides and
glycoproteins.
Frank P. Barry, Osiris Therapeutics, Inc.
Structure and Distribution of the N-linked Oligosacharides of Cartilage
Oligomeric Matrix Protein (COMP)
R. Reid Townsend, Univ. of California, Berkeley
Structural Studies on the N-linked Oligosaccharides of the Gastric H,K
Atpase: A Paradigm for the Analysis of Sample Limited Membrane
Glycoproteins
Joseph Zaia, Osiris Therapeutics, Inc.
Practical Analysis of Glycoproteins using Matrix Assisted Laser
Desorption/Ionization Time-of-Flight Mass Spectrometry
Bruce B. Reinhold, Boston Univ. Sch. of Med.
Practical Aspects of Carbohydrate Analysis by Electrospray Ionization
Mass
Spectrometry
2:00 PM - 4:00 PM
CONCURRENT TUTORIAL SESSIONS
TUTORIAL: SEQUENCE DATABASE SEARCHES
CONSTELLATION BALLROOM F
Chairs: Hugh B. Nicholas Jr., Pittsburgh Supercomputing Ctr.
Hugh B. Nicholas Jr., Pittsburgh Supercomputing Ctr.
Performing and Interpreting Sequence Database Searches
David W. Deerfield II, Pittsburgh Supercomputing Ctr.
Using Network Resources to Carry Out Sequence Database Searches
TUTORIAL: DNA SEQUENCE ANALYSIS
CONSTELLATION BALLROOM E
Chair: Harold G. Hills, Iowa State Univ.
Harold G. Hills, Iowa State Univ., Marcia D. Elliot, Univ. of Nebraska
and
Eleanor K. Spicer, Med. Univ. of South Carolina
TUTORIAL: PEPTIDES FROM HELL
CONSTELLATION BALLROOM D
Chair: John S. McMurray, Univ. of Texas, M.D. Anderson Cancer Ctr.
The request for a peptide comes into the lab. You tell the customer, or
your
friend and collaborator, "No problem. We'll have this compound made for
you
in no time!" Three weeks later all you have is an HPLC profile that looks
like a comb and one of the peaks may be the correct peptide. In this
tutorial
we'll discuss peptide synthesis projects that have gone completely wrong
and
what we have done to solve the problems.
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Kristin Mirabal, Meeting Manager
kmirabal@faseb.org
301/530-7010
Fax: 301/530-7014
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