Date: Thu, 09 Jan 1997 14:59:29 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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Date: Mon, 10 Jun 1996 19:06:32 -0600 (MDT)
From: Ed Meenen <emeenen@howard.genetics.utah.edu>
Subject: Re: DNASYN: LV 40nmol syn
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Old-To: Barbara Revere <revereb@ava.BCC.ORST.EDU>
Barbara
I run a DNA/RNA synthesis facility and do approximately 5-6,000oligos/year.
Approximately 75% are 40nmol. I have been using the LV columns for more
than 6 months and see no difference between std and LV 40 nmol columns.
I have made oligoe out to approximately 130 bases. Both strands which
were gel purified and cloned. 9 out of 10 clones sequenced gave the
correct sequence. I have found the there is a direction the LV columns
and need to be put on 39x synthesizers label up. PE/ABD better not change
the direction of these columns. I have also move to using the 200nmol LV
columns. The LV columns are a big savings for some reagents. Acetonitrile
is not saved to a large extent only a small amount. There is also
a benifit of less waste (a cost savings). I used only the standard cycles
from the sales persons.
Ed Meenen
HHMI
Universisty of Utah
Salt Lake City, UT 84112