Date: Thu, 09 Jan 1997 15:12:25 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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Date: Wed, 17 Jul 1996 07:07:05 +0800 (U)
From: "Brasseur.Mike" <brasseur@rapids.Tularik.com>
Subject: Re: CZE of oligos
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Steve French asked about a reliable method to CE oligos:
I have a BioRad Biofocus 3000 and over the last 4 years, have analyzed over 7000
synthetic oligos using their non-gel sieving buffer (CE oligonucleotide Buffer
148-5026). This is a replaceable polymer and is used with their coated capillary.
This method allows the replacement of the buffer after each run and produces
reproducible separations every bit as good as gel-filled capillaries. The
columns are much easier to deal with being you can leave them empty after the
last run . Gel-filled capillaries can be more difficult to deal with because you
must keep the ends wet at all times or you run the risk of having the ends dry
out which can lead to a dead column. I have gotten over a 1000 runs on a number
of my columns without a degradation of separation.
I hope this was helpful.
Mike Brasseur
Tularik Inc.
415-828-4404
brasseur@tularik.com