DNA Synthesis

Terry Mulcahy 272-5792; fax 272-9107 (TMULCAHY@medusa.unm.edu)
Thu, 09 Jan 1997 15:13:45 -0700 (MST)

Date: Thu, 09 Jan 1997 15:13:45 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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From: COREY.LEVENSON@roche.com
Subject: Re: Nearest-Neighbour thermodynamics
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Hi Kevin,

Here are a few recent references you might find helpful (particularly the
middle one):

Kallansrud, G., and B. Ward. "A comparison of measured and calculated single-
and double-stranded oligodeoxynucleotide extinction coefficients." Anal Biochem
236.1 (1996): 134-138.

SantaLucia, J., Jr., H. T. Allawi, and P. A. Seneviratne. "Improved nearest-
neighbor parameters for predicting DNA duplex stability." Biochemistry 35
(1996): 3555-3562.

Sugimoto, N., et al. "RNA/DNA hybrid duplexes with identical nearest-neighbor
base-pairs have identical stability." FEBS Lett 354.1 (1994): 74-78.

Good luck,

Corey Levenson
Roche Molecular Systems
Alameda CA

>Dear ABRF's,

>I have seen many references to the papers of 1986 for calculating free
>energy, enthalpies, Tm's etc. using the nearest neighbour method. I presume
>that there have been subsequent publications on this subject. Does anyone
>have the latest reference information on this subject?

>Thanks once again,
>Kevin
___________________________________________________________________________
>Kevin McDermott
>Core Facility for Protein/DNA Chemistry
>Department of Biochemistry
>Queen's University
>Kingston,ON K7L 3N6
>Canada