Date: Thu, 09 Jan 1997 15:14:19 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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Date: Fri, 13 Sep 1996 10:48:44 +0000
From: cook@MIT.EDU (richard f. cook)
Subject: Re: DNAsyn: vials and labels
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Richard,
John Flory at Yale helped us in streamlining our oligo operation and one
of his best suggestions was to do all manipulations in the 4cc vial that
screws into the synthesizer. We sent our speedvac 13mm tube rotor to
Savant along with a 4cc vial with a label on it and they nicely drilled
out the holes to fit the dna vials.
We deprotect in the vials, put them in a Pierce evaporator to evaporate
most of the NH4OH and then go directly to the speedvac. They come out of
the speedvac and go to the researcher. Breakage has never been a
problem.
The same program we use to track lab work, File Maker Pro, is used to
print out the labels.
John also suggested All-Pack (Pittsburgh, PA) Teflon caps for the 4cc
vials which cut the cost per cap from $0.40 to $0.12! They work very
well.
Regards,
Dick Cook
MIT