Date: Thu, 09 Jan 1997 15:16:30 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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Date: Fri, 18 Oct 1996 16:22:53 -0400 (EDT)
From: Christopher O Conway <coconway@welchlink.welch.jhu.edu>
Subject: Re: Oligo deprotection
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On Fri, 18 Oct 1996 SGW@shcc.org wrote:
> Regarding post-cleavage deprotection at 55 degrees, how long is too long? Can
> they stay at 55 for 24 or more hours without degrading? Thanks.
>
> Stephanie Willis
> Shriners Hospital Research Unit
> Portland, OR
>
>
I was originally told by ABI that 24 hrs was the maximum you should let
the oligos sit at 55C. Up to 24 was OK. 24 and ten seconds... too long.
Good luck
Christopher O Conway
Lab Coordinator DNA Analysis Facility
Genetics Resources CORE Facility
Johns Hopkins University
Baltimore, Maryland USA