Date: Thu, 09 Jan 1997 15:22:12 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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Date: Sat, 19 Oct 1996 12:04:37 -0600 (MDT)
From: "Richard T. Pon" <rtpon@acs.ucalgary.ca>
Subject: Re: Oligo deprotection
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On Fri, 18 Oct 1996 SGW@shcc.org wrote:
>
> Regarding post-cleavage deprotection at 55 degrees, how long is too long? Can
> they stay at 55 for 24 or more hours without degrading? Thanks.
>
> Stephanie Willis
> Shriners Hospital Research Unit
> Portland, OR
>
>
Hi
I don't know of any literature references but we regularly leave
our samples in the heating block over both 2 and 3 day weekends and
haven't notice any problems. We also deliberately double deprotect our
very long oligo's by heating them overnight, evaporating off the ammonium
hydroxide, adding fresh ammonium hydroxide and heating them for a second
night.
Richard T. Pon, Director E-mail: RTPon@acs.ucalgary.ca
University Core DNA Services Phone: (403) 220-4277
University Of Calgary Fax: (403) 283-4907
Please direct DNA synthesis or sequencing orders to DNALAB@acs.ucalgary.ca