Date: Thu, 09 Jan 1997 15:31:52 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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Date: Mon, 28 Oct 1996 08:03:13 -0500 (EST)
From: Lawrence Udell <ludell@lynx.dac.neu.edu>
Subject: Trityl Monitoring
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I used an ABI 392/4 for study earlier this year and saw many of the same
"traits" with our trityl monitor. First, check to see how many cycles
you are averaging over. We would average over 4 to 5 cycles which makes
for some wierd jumps, especially in the beginning. If you suspect an
instrument problem, check the restrictors - they get even more "restricted"
with use, especially if you sieve your solvents (use the sieve bags
available from Prime Synthesis to avoid sieve dust clogging). Partially
clogged restrictors seem to cause erratic, small yield dips and poor
column to column reproducibility. BTW - does anyone know how to clean
these things once they start to clog? Replacing them can get expensive.