Date: Thu, 09 Jan 1997 15:32:34 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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Date: Mon, 28 Oct 1996 11:32:20 -0500 (EST)
From: Stephen.A.Bobin@Dartmouth.EDU (Stephen A. Bobin)
Subject: Re: Trityl monitoring
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What size synthesis are you doing? We find it works okay for 0.2 micromole,
which I'm given to understand (ABI) is what it was intended for, but 40nmole
gives us the same sort of erratic readings you are talking about. We basiclly
use it as a kind of on/off switch or rather a yes/no switch. If a synthesis is
really bad <85% we discontinue it. It's been my experience with oligo
synthesis that it ether works or it does not. I leave everything in between to
the mass spec guys (we don't have one). When it does not work it's usually
major disaster; operator and/or reagent related rarely the 394 itself. So, I
don't think it's useless it's just not good for defending the quality of a
40nmole synthesis. If one of our oligos does not work we re-make it after
checking for design flaws and grilling the individual about their experiment.
If it fails after that "sorry we can't help you, no charge". Just out of
curiosity how much crude OD do you get from a 40nmole synthesis of a 20mer?
Steve