Date: Thu, 09 Jan 1997 15:34:56 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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Date: Mon, 28 Oct 1996 09:54:33 -0800 (PST)
From: Barbara Revere <revereb@ava.BCC.ORST.EDU>
Subject: Re: Trityl Monitoring
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Old-To: Lawrence Udell <ludell@lynx.dac.neu.edu>
We clean our point restrictors in 100% nitric acid.
1. Rinse restrictors in acetonitrile
2. Rinse in pure water
3. Soak in 100% nitric acid over night
4. Rinse in pure water--several changes
5. Sonicate for 1 hour in pure water
6. Dry and store
We do this once a week, and whenever we get a low ASWY, < 98%.
We used to check the flow of the restrictors, by weight, after this
procedure, but never found a problem. Now we check by sight---all 4
columns 'appear' to flow at the same rate during a function 42.
Barbara Revere *revereb@bcc.orst.edu
Center for Gene Research and Biotechnology
Oregon State University, Corvallis, OR 97331
Phone: 541-737-3413 Fax: 541-737-3045
On Mon, 28 Oct 1996, Lawrence Udell wrote:
>
> I used an ABI 392/4 for study earlier this year and saw many of the same
> "traits" with our trityl monitor. First, check to see how many cycles
> you are averaging over. We would average over 4 to 5 cycles which makes
> for some wierd jumps, especially in the beginning. If you suspect an
> instrument problem, check the restrictors - they get even more "restricted"
> with use, especially if you sieve your solvents (use the sieve bags
> available from Prime Synthesis to avoid sieve dust clogging). Partially
> clogged restrictors seem to cause erratic, small yield dips and poor
> column to column reproducibility. BTW - does anyone know how to clean
> these things once they start to clog? Replacing them can get expensive.
>
>