Date: Thu, 09 Jan 1997 15:35:51 -0700 (MST)
From: "Terry Mulcahy 272-5792; fax 272-9107" <TMULCAHY@medusa.unm.edu>
Subject: DNA Synthesis
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Date: Tue, 29 Oct 1996 11:26:13 +0000 (GMT)
From: Ken Taylor <KTAYLO@scri.sari.ac.uk>
Subject: DNA Synth
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Hi,
to chip in about Terry Mulcahy's queries on trityl monitoring.
I have this facility on my 394 and see quite a bit of variation as
well. I've also noticed that the overall percentage yield bears
little relation to the quantity of crude oligo I recover.
However, its major benefit is in being able to interrupt synthesis
if the efficiency falls below a set value. Any steps below 85%
generally indicate problems and every time it's interrupted there has
been a genuine problem. This facility has saved me quite a bit in
lost reagents and time. I wouldn't describe it as useless.
Ken.
Ken Taylor.
Peptide and DNA Services.
Scottish Crop Research Institute, Invergowrie, Dundee.
Phone 01382 56 27 31 Fax 01382 56 24 26
WWW URL http://www.scri.sari.ac.uk/