From: Jim Sparrow <jsparrow@athero.med.bcm.tmc.edu>
Subject: RE: SEQ - Procise
Date: Thu, 09 Jan 97 14:16:00 C
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Mark,
I have not done resin bound peptides on the Procise but have found that with
the 477 that sequencing works best if the resin is not washed with heptane
or ethyl acetate, i.e., only use butyl chloride for washes and extraction.
No temperature changes are required and the yields are higher and the
carryover between cycles is greatly reduced. The same principles should
apply to the Procise since the non polar solvents shrink the resin beads and
subsequently trap the derivatives or prevent access of reagents. With FMOC
chemistry these problems are not so much a consideration since the peptide
is cleaved from the resin after the first cycle or two. Loss of peptide
from the filter may become a problem. Jim Sparrow
-------------------------------------------------------
Dr. James T. Sparrow
Dept. of Medicine
Baylor College of Medicine
One Baylor Plaza
Houston, TX 77030
Phone: 713 798-4156
FAX: 713 798-4121
email: jsparrow@bcm.tmc.edu
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From: abrf-request
To: Recipients of ABRF List
Subject: SEQ - Procise
Date: Thursday, January 09, 1997 10:37AM
Hello! Can anyone comment on doing resin bound peptide sequencing on a
Procise.
Thank you for your time on this matter. Mark.
Mark W. Crankshaw
Protein & Nucleic Acid Chemistry Laboratories
Washington University School of Medicine
voice: (314) 362-0285
FAX: (314) 362-4698
email: crank@pharmdec.wustl.edu