Message-Id: <199701141658.LAA09770@post.aecom.yu.edu>
Subject: Lost Protein
Date: Tue, 14 Jan 97 11:19:36 -0500
From: Rick Edmondson <Ricky@ACS.TAMU.EDU>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
-- [ From: Rick Edmondson * EMC.Ver #2.5.02 ] --
Dear ABRF'ers
I am trying to analyze a protein (conc~0.1 uM) by MALDI-TOF, I routinely
analyze proteins applying low fmol amounts, but I am having problems with
this 14kDa protein. The protein was isolated a few months ago and the hplc
fractions have been frozen since then in microcentrifuge tubes (polyethylene
or polycarbonate). My question is, from a dilute solution (~0.1 uM) how
much protein will "stick" to the walls of the container after a few months
from an unbuffered solution? I didn't do the isolation, but I was told that
a faint silver stained band was observed from the HPLC fraction (several
months ago) so evidently there was protein there, but I can't see any now.
Any suggestions?
thanks in advance
Rick
-- Rick Edmondson Laboratory for Biological Mass Spectrometry Department of Chemistry Texas A&M University College Station, TX 77843phone 409-845-0613 fax 409-845-9485 email Ricky@ACS.TAMU.EDU