Date: Tue, 14 Jan 1997 12:13:54 -0600
From: lsteinke@molbio.unmc.edu (Laurey Steinke)
Message-Id: <199701141813.MAA11761@molbio.unmc.edu>
Subject: Re: K/K'K"
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Dear Ron, In the one case where I have seen the K'K" ( or at least think it
is the same thing although they are eluting earlier in my gradient) the sample
had been run on an HPLC to purify it. It was a synthetic peptide.
-laurey
Weather Inconsequential: We lived through the bitter cold of the weekend and
have received some light snow. The world is beautiful just now.
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>From RLNiece@aol.com Tue Jan 14 10:40 CST 1997
From: RLNiece@aol.com
To: lsteinke@unmc.edu
Subject: K/K'K"
Recalling my comment recently about killing K's... We have some suspicion
that the HPLC solvents from the instruments we have been using to purify
proteins and peptides may be the cause. We will be examining it over the
next few days.
The initial observation is that most (we are still searching for contrary
examples) cases where K is very low and the other two peaks appear have been
run on one of the HPLC's before sequencing. We will let you know of the
outcome.
If you have contrary examples, would you let us know. If you have passed the
information on to others, would you querry them?
Thanks.
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