From: bcdchin@muccmail.missouri.edu
Date: Tue, 14 Jan 97 16:40:51 CST
Message-Id: <9700148532.AA853281811@muccmail.missouri.edu>
Subject: Re: Lost Protein
To: Recipients of ABRF List <abrf@aecom.yu.edu>
As a rule of thumb, when the solution is below 1 ug/ml the protein or peptides
tends to stick to the tube. However, there are many exceptions and you might be
dealing with one. Usually the protein will not stick in the frozen state, but
many come out of solution, ie ppt. For sticking try to get it off the tube with
either 90% TFA or formic acid or slight basic solutions.
David Chin
UMC PRotein Core
-------------------
In response to:
-- [ From: Rick Edmondson * EMC.Ver #2.5.02 ] --
Dear ABRF'ers
I am trying to analyze a protein (conc~0.1 uM) by MALDI-TOF, I routinely
analyze proteins applying low fmol amounts, but I am having problems with
this 14kDa protein. The protein was isolated a few months ago and the hplc
fractions have been frozen since then in microcentrifuge tubes (polyethylene
or polycarbonate). My question is, from a dilute solution (~0.1 uM) how
much protein will "stick" to the walls of the container after a few months
from an unbuffered solution? I didn't do the isolation, but I was told that
a faint silver stained band was observed from the HPLC fraction (several
months ago) so evidently there was protein there, but I can't see any now.
Any suggestions?
thanks in advance
Rick
-- Rick Edmondson Laboratory for Biological Mass Spectrometry Department of Chemistry Texas A&M University College Station, TX 77843 phone 409-845-0613 fax 409-845-9485 email Ricky@ACS.TAMU.EDU