From: JWLEONE@am.pnu.com
Date: Tue, 14 Jan 1997 18:12:05 -0500
Message-Id: <0000568F.1444@am.pnu.com>
Subject: Re: ProtSeq - 494 ProciseHT problem
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Sherry,
I sympathize with your problem. If you are not getting a sample loop full
sensor error the problem must be somewhere other than the injector. The
flask dry downs and bubble look OK - the problem must be upstream -
variable ATZ extraction??-who knows??-all the Procise bells and whistles
indicate no problem. Steve Tindall (Hello Steve) may be right about the
variable S4 resolubilization, but not because of load volumes since these
are sensor monitored. Having encountered this frustrating problem I noticed
that if I repositioned the flask transfer tube just slightly higher in the
flask, the variance in yields was eliminated. Why this helped-I am not sure
- but it worked. I have also been experimenting with a slight diagonal cut
for the end of the flask transfer tube and this seems to be working fine.
Let me know if this eliminates your problem.
Joe
a midwestern pseudo-lurker
P.S. I've noticed quite a few different "species" of lurkers on this
bulletin board - have any attempts been made to classify us - possible
phylogenic tree??
jwleone
Pharmacia & Upjohn
Biochemistry
Kalamazoo,MI
joseph.w.leone@am.pnu.com
______________________________ Reply Separator _________________________________
Subject: ProtSeq - 494 ProciseHT problem
Author: sparky@umich.edu at INTERNET
Date: 1/13/97 3:47 PM
Hello-
We had our Procise installed in September, 1996. Shortly after, we
noticed a variance from cycle to cycle in amino acid pmol yields; in
one cycle all yields would be up and the next, all yields would be
down. To try and troubleshoot this problem, we added an internal
standard to the amino acid standard and also to all sequencing
cycles. As we saw previously, when the yields go up the internal
standard goes up too, and vice versa. ABI seems to think that this
is not an injection problem, however, we aren't convinced. As you
can imagine, this makes sequence calling somewhat difficult.
Has anyone out there experienced this sort of problem? If so, what
have you done to solve the problem.
Thank you in advance-
Sherry Williams
University of Michigan
Protein and Carbohydrate Structure Facility
Ann Arbor, MI
sparky@umich.edu