Date: Wed, 15 Jan 97 07:55:27 -0500
From: "Thomas J Miller" <millertj@a1.esvax.umc.dupont.com>
Message-Id: <52318051107991/5041849@ESVAX>
Subject: RE: PSeq line transfers
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Hello. I'll admit that I see once in awhile the same nice call and then
sudden and dramatic drop, followed by a rebound. I usually contribute this
to a bad delivery and do my visual checks for deliveries and transfers.
My Beckman service engineer developed a technique where he draws a
capillary tip on the transfer vial pickup tube, makes his cut and then
carefully positions the tube end just above the bottom of vial. It does an
excellent job of picking up the vial contents. I think the factory
technique was to do a square flush cut which naturally didn't pickup the
entire contents of the flask.
Regards, Tom.
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Thomas Miller, Macromolecular Analysis, Protein Sequencing
Dupont Co., POB 80328, Wilmington, DE 19880
Voice 302-695-1745
email: millertj@esvax.dnet.dupont.com
Opinions are my own and not of my employer.
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
ps: I was amazed to see the outpouring of postings regarding the
recent question on labs offering MS services. I had to exit my email
program on my Vax account, than came back to it a few minutes later.
All 10 new postings were labs answering the call for MS sample help.
That must be a record. Tom M.
===========================================================
joseph.w.leone wrote:
Sherry,
I sympathize with your problem. If you are not getting a sample loop full
sensor error the problem must be somewhere other than the injector. The
flask dry downs and bubble look OK - the problem must be upstream -
variable ATZ extraction??-who knows??-all the Procise bells and whistles
indicate no problem. Steve Tindall (Hello Steve) may be right about the
variable S4 resolubilization, but not because of load volumes since these
are sensor monitored. Having encountered this frustrating problem I noticed
that if I repositioned the flask transfer tube just slightly higher in the
flask, the variance in yields was eliminated. Why this helped-I am not sure
- but it worked. I have also been experimenting with a slight diagonal cut
for the end of the flask transfer tube and this seems to be working fine.
Let me know if this eliminates your problem.
jwleone
Pharmacia & Upjohn
Biochemistry
Kalamazoo,MI
joseph.w.leone@am.pnu.com