Date: Thu, 16 Jan 1997 12:10:56 -0600
Message-Id: <v01530507af051d4a0ae5@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: PepSyn: EDT/side products?
>Date: Wed, 14 Aug 1996 08:53:09 +0200
>From: "assaf friedler" <fasaf@leonardo.ls.huji.ac.il>
>Old-To: BRESLAV@POSTBOX.CSI.CUNY.EDU, abrf@aecom.yu.edu
>Subject: Re: PepSyn: EDT/side products?
>Mime-Version: 1.0
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Sender: Association of Biomolecular Resource Facilities
><abrf-request@aecom.yu.edu>
>Precedence: bulk
>
>Responding to the message of Tue, 13 Aug 1996 17:35:28 -0400
>from "Michael Breslav" <BRESLAV@POSTBOX.CSI.CUNY.EDU>:
>>
>> Hello:
>> We know, EDT is a conventional scavenger used with TFA
>> for the cleavage and deprotection of peptides assembled on HMP resin.
>> Now I am interested in learning side reactions involving EDT.
>> I would appreciate any reference on the formation of EDT derived
>> peptides - side products - during TFA cleavage.
>> Thanks,
>> Michael Breslav
>Hello,
>
>I used to work with EDT as a scavenger in TFA and HF cleavages. When I
>cleaved a cys-containing peptide in HF using EDT as a scavenger I got
>after the usual workup a solid which did not dissolve in any solvent. We
>think that what occured there is polymerization of EDT with the peptide.
>When we used thioanisole instead of EDT the product was soluble in water
>and in 30% acetic acid.
>
>Hope that helps,
>
>Assaf Friedler
>Dept. of Organic Chemistry
>The Hebrew University of Jerusalem
>Israel
>
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110