Date: Thu, 16 Jan 1997 12:11:17 -0600
Message-Id: <v01530508af051d651127@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: PepSyn: EDT/side products?
>Date: Wed, 14 Aug 1996 12:37:16 -0400 (EDT)
>From: "Angela c. Murphy" <acmurphy@helix.nih.gov>
>Old-To: abrf@aecom.yu.edu
>Subject: Re: PepSyn: EDT/side products?
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>Precedence: bulk
>
>A few years ago, I read an article that mentioned dithioketal formation
>between EDT and tryptophan. The cleavage was performed at 50 degrees C.,
>however, and later, other scavengers were used in addition to EDT, which
>minimized the problem. The paper is by Peter Sieber (1987) Tet. Lett.,
>Vol. 28, #15, pp. 1637-1640. Hope this helps.
>Angela C. Murphy
>
>On Tue, 13 Aug 1996, Michael Breslav wrote:
>
>> Hello:
>> We know, EDT is a conventional scavenger used with TFA
>> for the cleavage and deprotection of peptides assembled on HMP resin.
>> Now I am interested in learning side reactions involving EDT.
>> I would appreciate any reference on the formation of EDT derived
>> peptides - side products - during TFA cleavage.
>> Thanks,
>> Michael Breslav
>> College of Staten Island, NY
>>
>
>*******************************************
> Angela C. Murphy, Chemist
> Lab. of Cell Biology, NHLBI, NIH
> 3 Center Drive, MSC 0301, Rm. B1-22
> 9000 Rockville Pike
> Bethesda, MD 20892-0301 USA
> tel.: (301) 496-2324
> fax: (301) 402-1519
> email: acmurphy@helix.nih.gov
>*******************************************
>
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110