Date: Thu, 16 Jan 1997 12:11:48 -0600
Message-Id: <v01530509af051d841889@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: Ether precipitation after TFA cleavage
>From: "Lefebvre, Jean" <JEAN.LEFEBVRE@nrc.ca>
>Old-To: ABRF <abrf@aecom.yu.edu>
>Subject: Ether precipitation after TFA cleavage
>Date: Tue, 24 Sep 96 09:42:00 EDT
>Encoding: 15 TEXT
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Sender: Association of Biomolecular Resource Facilities
><abrf-request@aecom.yu.edu>
>Precedence: bulk
>
>Hi,
>
>After TFA cleavage, our peptides are precipitated in Ethyl Ether.
>We want to avoid to filter those solutions because it is very long since we
>do about 24 at a time.
>We think about centrifugatrion but Ether in centrifuge can explode with a
>spark.
>Does anyone have a suggestion...
>
>Jean Lefebvre
>BRI/NRC
>Montreal,P.Quebec
>Canada
>
>E-Mail: lefebvre@biotech.lan.nrc.ca
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110