Date: Thu, 16 Jan 1997 12:12:12 -0600
Message-Id: <v0153050aaf051d9c1e0a@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: Ether precipitation after TFA cleavage
>Date: Tue, 24 Sep 1996 15:33:03 -0400 (EDT)
>X-Sender: jfarmar@server.nybc.org
>Mime-Version: 1.0
>Old-To: abrf@aecom.yu.edu
>From: Jim Farmar <jfarmar@nybc.org>
>Subject: Re: Ether precipitation after TFA cleavage
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Sender: Association of Biomolecular Resource Facilities
><abrf-request@aecom.yu.edu>
>Precedence: bulk
>
>At 09:42 AM 9/24/96 EDT, you wrote:
>>Hi,
>>
>>After TFA cleavage, our peptides are precipitated in Ethyl Ether.
>>We want to avoid to filter those solutions because it is very long since we
>>do about 24 at a time.
>>We think about centrifugatrion but Ether in centrifuge can explode with a
>>spark.
>>Does anyone have a suggestion...
>
>We have used 15 or 50 mL polypropylene centrifuge tubes to spin down our
>precipitates for several years without a problem. Make certain that the
>caps are on securely and leave a few mLs of air for when the tube tilts in
>the centrifuge (e.g., 12 mL of liquid in the 15 mL tube). Once in a while a
>cap will "strip" but you can take another cap and continue.
>You still may want to place your centrifuge in a hood.
>
>Regards,
>Dr. Jim Farmar
>MicroChemistry
>L.F. Kimball Research Institute
>New York Blood Center
>310 East 67th Street
>New York, NY 10021 USA
>212 570-3128
>212 570-3195 (fax)
>jfarmar@server.nybc.org
>
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110