Date: Thu, 16 Jan 1997 12:14:34 -0600
Message-Id: <v0153050caf051e2e4059@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: Peptide with many Arg's
>From: matherj@rockvax.rockefeller.edu
>Mime-Version: 1.0
>Date: Tue, 02 Jul 96 08:54:57 -0500
>Old-To: abrf@aecom.yu.edu
>Reply-To: matherj@rockvax.rockefeller.edu
>Subject: Peptide with many Arg's
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Sender: Association of Biomolecular Resource Facilities
><abrf-request@aecom.yu.edu>
>Precedence: bulk
>
>I need to synthesize a 27-mer peptide that has 9 Arg residues. Does anyone
>have any suggesstions on how to improve my chances for success. The rest of
>the peptide is largely Glu, Lys, and Leu.
>
>I will be using FMOC chemistry and TFA cleavage/deprotection. I plan to
>extend the cleavage time to around 4 hr and use the standard King mixture
>of TFA/phenol/ethanedithiol/thioanisole/water.
>
>TIA,
>Jeff Mathers
>Peptide Synthesis Manager
>The Protein/DNA Technology Center
>The Rockefeller University
>http://pdtc.rockefeller.edu
>
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110