Date: Thu, 16 Jan 1997 12:15:25 -0600
Message-Id: <v0153050eaf051e584a61@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: Peptide with many Arg's
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>Date: Thu, 04 Jul 1996 09:09:30 +1000
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>From: auspep@c031.aone.net.au (Auspep Pty Ltd)
>Subject: Re: Peptide with many Arg's
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>>I need to synthesize a 27-mer peptide that has 9 Arg residues. Does anyone
>>have any suggesstions on how to improve my chances for success. The rest of
>>the peptide is largely Glu, Lys, and Leu.
>>
>>I will be using FMOC chemistry and TFA cleavage/deprotection. I plan to
>>extend the cleavage time to around 4 hr and use the standard King mixture
>>of TFA/phenol/ethanedithiol/thioanisole/water.
>>
>>TIA,
>>Jeff Mathers
>>Peptide Synthesis Manager
>>The Protein/DNA Technology Center
>>The Rockefeller University
>>http://pdtc.rockefeller.edu
>>
>>
>>
>Here at Auspep, we've made an 18mer with 11 Arg residues, (Mtr-protected),
>of which 6 were adjacent. We routinely use 5% phenol/5% EDT in TFA
>overnight at RT. Naturally, we would only recommend your doing so
>initially on a sample amount of resin. If cost is not a consideration,
>Arg(pmc) would be protection of chioce. Though we do large amounts of HF
>cleavages routinely, we wouldn't bother with it in such a case unless other
>methods proved unsuccessful. However you choose to go, best of luck!
>The Guys & Dolls from Auspep (Downunder)
>
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110