Date: Thu, 16 Jan 1997 12:39:19 -0600
Message-Id: <v01530519af0523f49bc3@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: PepSyn: cyclized.
>From: Haaseth@brcf.med.umich.edu
>Date: Thu, 27 Jun 96 17:30:04 EST
>Old-To: abrf@aecom.yu.edu
>Subject: Re: PepSyn: cyclized.
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Sender: Association of Biomolecular Resource Facilities
><abrf-request@aecom.yu.edu>
>Precedence: bulk
>
>
>
>> C K G D K G D K G D
>
>> He would like it cyclized n-terminus to c-terminus.
>
>Lisa,
>
>You can do this with Fmoc-Asp(PEG-PS)-OAl resin from Perserptive Biosystems.
>Catalog # GEN9111310. After building up your peptide, remove the allyl group
>on the C-alpha of the Asp, remove the N-terminal Fmoc on the Cys, activate to
>cyclize on the resin, then cleave. Use the procedure published by Kates,
>et al
>[ (Analytical Biochemistry, 212, 303-310, (1993)] to remove the allyl.
>
>Good Luck.
>
>Ron Haaseth
>Protein Structure Facility
>University of Michigan
>We're on the WEB: http://brcf.med.umich.edu/
>
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110