Message-Id: <9701161509.AA23387@ibch.siobc.ras.ru>
From: "Vladimir Titov" <titov@ibch>
Date: Thu, 16 Jan 1997 18:10:35 +0300
Subject: Re: separation
In-Reply-To: <199701160949.EAA02032@post.aecom.yu.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
> Dear ABRF.
> I'm unable to isolate the peptides from NMP solvent. I can't use in HPLC
> for separation.
> Does anyone know some othar metods? Please assist me in this.
>
> Thanks for your help.
> Efron Leah
> The Hebrew University of Jerusalem
> Dep. of Organic Chemistry,Jerusalem
> Tel.972 2 6586181
> e-mail: leah@vms.huji.ac.il
Try ether precipitation (if a solution is not VERY diluted). Use plenty of
DRY ether. If it does not help, try to lyophilize a dilute aqueous solution
(it works with DMSO). If the peptide is well soluble in water, you can try
to extract NMP to an organic solvent. As a last resort: dilute with water
and apply on an HPLC column using an injection pump. The NMP would be
flushed away.
Vladimir Titov
MailTo:titov@ibch.siobc.ras.ru
http://titov.siobc.ras.ru