From: "Haydn Ball" <hlb@prion.ucsf.edu>
Message-Id: <9701161742.ZM12975@prion.ucsf.edu>
Date: Thu, 16 Jan 1997 17:42:11 -0800
Subject: RP-HPLC at basic pH
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Hello everyone,
I have a question concerning the reversed-phase purification of synthetic
peptides that have a strong tendancy to aggregate at the buffer pH most
commonly used for HPLC ie TFA/pH ~2. Does anyone have experience of separation
at higher pH's and can advise me on the types of commercially available
columns, given that silica based media are not stable at pH greater than about
8-9. Also any suggestions as to the buffer systems that are effective would be
greatly appreciated.
Many thanks in advance.
Haydn Ball
hlb@prion.ucsf.edu
Dept. of Neurology,
University of San Francisco
San Francisco
CA 94143-0518