Date: Fri, 17 Jan 1997 01:17:52 GMT
Message-Id: <v02140b03af040fac1f39@[134.197.50.216]>
From: schooley@unr.edu (David A. Schooley)
Subject: Re: pepsyn
To: Recipients of ABRF List <abrf@aecom.yu.edu>
At 10:05 AM 1/15/97, Leo Benoiton quaintly wrote:
>Dear David Schooley:
>I am sorry to advise that I submit you really did put your foot in it, as
>the expression goes.
>All Boc-, Z and Fmoc-pyroglutamic acid derivatives indeed do exist, and
Yes, Leo, and I managed to do it right when the server burped the message
SIX times to all members (although James Farmars unrelated message got
repeated nine times by my count). Thanks to the subsequent responses that
it is solely for the reason of solubility- I couldn't conceive of a
possible synthetic reason like preventing aspartimide formation. We are
just faced with synthesizing our first peptide terminating in pGlu, and
the pure (S)-isomer is available from Aldrich for $34 per MOLE, a whole lot
cheaper than ANY of the FMOC amino acids we usually buy!
NMP as used in the 431A is such a great solvent- surely someone
ought there must know if pGlu, devoid of protecting groups, has
appreciable solubility in NMP? I don't want to buy a rarely used
t-Boc-pGlu if the cheap stuff will do.
David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@med.unr.edu
tel: (702) 784-4136; fax (702) 784-1419