Date: 17 Jan 97 12:03:59 +0000
From: LARRY BRINK <larry.brink@smtp.regpha.com>
Subject: proseq
To: Recipients of ABRF List <abrf@aecom.yu.edu>
I'm fairly new at sequencing and would like to know if anyone has had problems sequencing proteins after electrblotting from TRICINE gels to PVDF membrane. How much should be loaded on the gel to get good sequencing results? Thanks for any help you can give me.
Larry Brink
Regeneron Pharmaceuticals, Inc
Rensselaer, New York
larry.brink@regpha.com