Date: Fri, 17 Jan 1997 19:36:50 -0500 (EST)
From: "Angela c. Murphy" <acmurphy@helix.nih.gov>
Subject: Re: RP-HPLC at basic pH
In-Reply-To: <9701161742.ZM12975@prion.ucsf.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
I have, so far, had very good luck with Vydac's "pH stable" C-8 columns,
228 TP. They are silica, with a polymer coating. I've used them with
10 mM ammonium bicarbonate, pH 7.8, and also have gone in the other
direction, to 0.5% TFA. If you want to have the full pH range, up to 14,
I'd recommend a fully polymeric column packing of the styrene-divinylben-
zene type. Hope this helps.
Angela C. Murphy
On Thu, 16 Jan 1997, Haydn Ball wrote:
> Hello everyone,
>
> I have a question concerning the reversed-phase purification of synthetic
> peptides that have a strong tendancy to aggregate at the buffer pH most
> commonly used for HPLC ie TFA/pH ~2. Does anyone have experience of separation
> at higher pH's and can advise me on the types of commercially available
> columns, given that silica based media are not stable at pH greater than about
> 8-9. Also any suggestions as to the buffer systems that are effective would be
> greatly appreciated.
> Many thanks in advance.
>
> Haydn Ball
>
> hlb@prion.ucsf.edu
> Dept. of Neurology,
> University of San Francisco
> San Francisco
> CA 94143-0518
>
*******************************************
Angela C. Murphy, Chemist
Lab. of Cell Biology, NHLBI, NIH
3 Center Drive, MSC 0301, Rm. B1-22
9000 Rockville Pike
Bethesda, MD 20892-0301 USA
tel.: (301) 496-2324
fax: (301) 402-1519
email: acmurphy@helix.nih.gov
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