Message-Id: <v0300780baf06925e6fcc@[129.186.10.114]>
Date: Sat, 18 Jan 1997 09:04:02 -0600
From: "Harold G. Hills" <hhills@iastate.edu>
Subject: DNASEQ - Seq Analysis V3.0
To: Recipients of ABRF List <abrf@aecom.yu.edu>
>
>Has anyone been routinely using version 3.0 of ABI's DNA
>Sequence Analysis software? Any reactions or comments
>that can be passed on to the group? I've played with it
>a little, and was rather disappointed with its much-
>vaunted lane tracking abilities. Faced with a gel having
>a few blank lanes, it refused to generate *any* tracking,
>even the poor lane tracking typical of V2.1.2 - all I got
>was useless straight-line tracks. Comments, anyone? any
>other flaws or nice features?
>
>Bob Lyons
>
>In response to Bob Lyons comments above. We have the xl upgrade installed
>on one of our 377's and have ordered it for our other two.
Yes, we are routinely using the software. We also have it installed on our
print server. I have been both amazed and disappointed in the ability to
track lanes. I will show a couple of examples at the ABRF DNA sequencing
tutorial. Moving the button to expand the gel certainly helped. I
cannot tell you how many times I closed a gel file when I was trying to
expand it on the old software. We have an extra copy on our print server.
The features for printing simply our life because we often want to print 3
gels in a row and can now load them all into sample manager and it prints
in the correct order without having to seperate the individual gels.
I have loaded a test with samples spread over the gel. For instance in
lanes 4,5; 10,11; 16,17, out to 48. It tracked the lanes perfectly.
Failure to track seems to happen most when samples do not work particularly
if it occurs in the first and last lane. I also found that it you indicate
sample in a lane and there is nothing in that lane than it tracks where it
finds info.
I wish there was more room on the gel sample sheet for comments because as
a core facility we like to make notes for samples that did not work or
worked poorly and what we will do when we rerun the samples.
Overall I'm satisfied.
Hal
.
Harold G. Hills, Ph.D. DNA Sequencing Specialist 515 294-9585
1184 Molecular Biology Building FAX 515 294-1597
Iowa State University
Ames, IA 50011-3260 hhills@iastate.edu
http://biotech.zool.iastate.edu/Biotech/Facilities/DSSF/homepage.html