From: Haaseth@brcf.med.umich.edu
Date: Mon, 20 Jan 97 13:32:29 EST
Message-Id: <9700208537.AA853795949@ccmailgw.brcf.med.umich.edu>
Subject: Re: pepsyn -- ninhydrin
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Paul,
You can make these reagents yourself, as we routinely do.
The recipe is in Stewart and Young. Not all of the final
reagent mixtures have long shelf-lives, but some of the
intermediate solutions can be stored.
Ron Haaseth
University of Michigan
PCSF
verdin@umich.edu
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>I have been using a ninhydrin kit to monitor coupling reactions in peptide
>synthesis. The kit is from Dupont NEN who no longer carries the kit.
>Does anyone know of a vendor who carries a ninhydrin kit. It consists of
>three reagents, ninhydrin in ethanol, pheonol in ethanol, and KCN in
>pyridine. Should I just make it myself?? Thanks in advance.
>Paul Motchnik
>XOMA Corp.
>Berkeley, CA