From: tossi@icgeb.trieste.it
Message-Id: <9701211732.AA07061@uts.univ.trieste.it>
Date: Tue, 21 Jan 1997 18:32:36
Subject: HPLC
To: Recipients of ABRF List <abrf@aecom.yu.edu>
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Hello everybody,
We have a synthetic amidated peptide which is giving us a problems in HPLC. The
crude material gave a fairly broad band with shoulders. We identified the
correct fraction and isolated it. The purified material now shows an odd
saw-tooth like background preceding a central peak with lots of shoulders. The
peptide also seems to ionize poorly in ES-MS, giving weak signals. The only
unusual aspect of this peptide for us is the presence of Cys at the C-terminal.
Does anyone have any suggestions?
Thanks Alex Tossi.
PS Thanks for the response to my HPLC equipment question last week.
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====================================================================== Dr. Alessandro Tossi Antiinfective peptides group Dept. of Biochemistry, Biophysics and Macromolecular Chemistry University of Trieste Phone -- 39 40 6763673; Fax -- 39 40 6763691 email: tossi@univ.trieste.it tossi@icgeb.trieste.it http://www.univ.trieste.it:80/~nirdbbcm/antimic.html =====================================================================
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