Message-Id: <v03007800af0a97dc1197@[155.52.82.136]>
In-Reply-To: <9701201042.AA22210@bccmsa.bc.ic.ac.uk>
Date: Tue, 21 Jan 1997 11:54:43 -0500
From: Paul Morrison <morrison@farber.harvard.edu>
Subject: Re:Re Seq.Analysis v3.0
To: Recipients of ABRF List <abrf@aecom.yu.edu>
At 5:53 AM -0500 1/18/97, Marc Woodland wrote:
> Hi happy sequencers,
....lots of kind words about Seq. Analysis 3.0 deleted...
> Other than that I like v3.0. Its Factura v2.0 that
>makes me froth at the mouth, chew the carpet!!
>
Has anyone figured out a use for Factura in a core facility setting? I
thought for sure the newest version would do something useful but I can't
figure out what.
What I would like it to do: Make a library of all the chunks of vector
sequence that are contiguous with all of the favorite polylinkers. Then
when Factura is run it easily identifies any vector sequence that is
present in a sequencing run. It should have the option not to delete
vector but it should high light the vector both on screen AND on the paper
chromatogram. You should have the option to not tell it which restriction
site is used in the polylinker. Because of this option it does not have to
be perfect to the nucleotide in selecting vector. Would this save anyone
some time by not manually editing vector sequence?
Because in a core facility one might have 36 (and now 48 or 64) different
vector parameters on a gel one does not want to have to keep telling
Factura what vectors or cloning sites are used. So if someone has figured
out how to do this could they send me their folder of "Vector Patterns".
I'm not chewing the carpet on this one because my expectations weren't that
high. I am chewing the carpet on copy protection serial numbers on Seq.
Analysis 3.0. What do they think I'm going to do? Run out and design an
automated sequencer that can be analysed with their software? Or is it just
to aggravate (or nickel and dime) a lab that might use more than one
computer to analyze data. The first time a computer crashes and I need to
reinstall the software but I've forgotten which serial number that computer
has I'm going to scream.
-Paul
Paul Morrison D1030
Molecular Biology Core Facilities
Dana-Farber Cancer Institute
44 Binney Street
Boston, MA 02115
p_morrison@dfci.harvard.edu
http://mbcf.dfci.harvard.edu
phone 617-632-3082
fax 617-632-4814