Date: Tue, 21 Jan 1997 15:32:50 -0800 (PST)
From: Ken Walsh <walsh@u.washington.edu>
Subject: Re: HPLC
In-Reply-To: <9701211732.AA07061@uts.univ.trieste.it>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Alex
Is there an Asn-Gly in the sequence? We have seen a sawtooth-like pattern
with one as it rearranged to the cyclic imide, then opened to the alpha
and beta Asp-Gly forms.
Ken Walsh
Dept Biochem
Univ of Washington
On Tue, 21 Jan 1997 tossi@icgeb.trieste.it wrote:
> Hello everybody,
>
> We have a synthetic amidated peptide which is giving us a problems in HPLC. The
> crude material gave a fairly broad band with shoulders. We identified the
> correct fraction and isolated it. The purified material now shows an odd
> saw-tooth like background preceding a central peak with lots of shoulders. The
> peptide also seems to ionize poorly in ES-MS, giving weak signals. The only
> unusual aspect of this peptide for us is the presence of Cys at the C-terminal.
> Does anyone have any suggestions?
>
> Thanks Alex Tossi.
>
>
>
>
> PS Thanks for the response to my HPLC equipment question last week.
>
> --
>