Date: Tue, 21 Jan 1997 19:27:26 -0500
From: HEMPEL@a.psc.edu
Message-Id: <970121192726.20209c91@CPWSCA.PSC.EDU>
Subject: Prot Seq: Astacus protease
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Hello all,
A number of years ago I used a protease from Astacus fluviatillus
(some sort of Crustacean, I think) which had the useful property of preference
for small side-chains like Ser, Thr, Gly and Ala, and it worked quite well.
(The downside was that these residues frequently cluster in sequences, and
it seemed to be a much better endopeptidase than amino- or carboxy-peptidase,
so ragged ends were not uncommon.)
Now, I have a need for this protease again, and was happy to find that it was
in the Serva catalog, only to find out that it was first out of stock, and then
on checking they told me it was no longer available.
Would anyone:
a) know of another supplier
b) know of another protease with similar specificity that is commercially
available
c) Know whether Prof. Robert Zwilling, Univ of Heidelberg, still makes this
enzyme. Last time it was a generous gift from him, but this was many years ago
and I have lost contact.
Thanks for any tips,
John Hempel
Department of Biol Sci
Univ Pittsburgh
(412) 624 0161, FAX (412) 624 4759