From: "Ken Taylor" <KTAYLO@scri.sari.ac.uk>
Date: Wed, 22 Jan 1997 10:14:13 GMT
Subject: Peptide hplc
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Alex,
I have recently been having problems with peptides having Cys at
the c-term. I also asked for advice in this group. (Thanks again to
all.)
The HPLC profile of my attempts looked like a cross section of the
Alps, with no particular predominant peak. Various suggestions were
made to me, the most common of which was to use 2-chlorotrityl resin
for the synthesis. I also got the following reference...
3-(1-Piperidinyl)alanine formation during the preparation of C-
terminal cysteine peptides with the Fmoc/t-Bu strategy"
in Letters in Peptide Science, 3 (1996), 157-166. Authors are J.
Lukszo, D. Patterson, F. Albericio and S. Kates.
......from Yvonne Angell.
In fact I got around the problem by re-making the sequence with Gly
at the c-term and Cys as residue 2. One lovely peak with one
much smaller lump as a crude product, starting from HMP resin with
normal F-moc synth and cleavage. I don't suppose you'll be lucky
enough to be able to get away with that, but ask.
Good luck. Ken.
Ken Taylor.
Peptide and DNA Services.
Scottish Crop Research Institute, Invergowrie, Dundee.
Phone 01382 56 27 31 Fax 01382 56 24 26
WWW URL http://www.scri.sari.ac.uk/