Date: Wed, 22 Jan 1997 09:32:27 -0600
Message-Id: <v01530500af0ce124ff71@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: cyclization of dissulfide peptide
>Date: Thu, 5 Dec 1996 11:52:03 -0500 (EST)
>From: Frances M Finn <fmf+@pitt.edu>
>X-Sender: fmf@unixs5.cis.pitt.edu
>Old-To: ABRF recipients <abrf@aecom.yu.edu>
>Subject: cyclization of dissulfide peptide
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>Dear All,
>
> We have recently been requested to make and cyclize the following
>peptide PCSAHDHRCLQKY-amide. We have tried both the DMSO and the
>ferricyanide procedures described by ABI which have worked well with other
>sequences. With this peptide we get approximately 5% cyclization and
>nothing that we have tried, including acylation of the N-terminal amino
>group before deblocking and cleaving has improved the yield. The peptide
>is being synthesized via the F-moc method. Incidentally the crude
>material shows 99% the correct molecular ion for the structure containing
>a completely deblocked peptide with (probably) two cysteines. I am not
>really positive that the two cys's are reduced but reducing them before
>cyclization has no imporved the situation.
>
> Does anyone have information that might be of help here?
>
>Frances Finn
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110