Date: Wed, 22 Jan 1997 09:34:56 -0600
Message-Id: <v01530504af0ce1b52166@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: Re: Proline cis-trans isomerism
>Comments: Authenticated sender is <titov@ibch.siobc.ras.ru>
>From: "Vladimir Titov" <titov@ibch.siobc.ras.ru>
>Organization: Shemyakin-Ovchinnikov Institute of bioorgan
>Old-To: abrf@aecom.yu.edu
>Date: Wed, 4 Dec 1996 15:28:39 +0300
>Mime-Version: 1.0
>Subject: Re: Proline cis-trans isomerism
>Priority: normal
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Sender: Association of Biomolecular Resource Facilities
><abrf-request@aecom.yu.edu>
>Precedence: bulk
>
>> We are working with a binding protein that recognizes hydrophobic sequences
>> that include proline residues (eg., VGVAPG repeats). We have found that
>> the binding protein is stereo specific in that it does not recognize
>> peptides where the x-P bond is in the cis configuration. Does anyone know
>> what percentage of X-P bonds assume the cis isomer when one synthesizes a
>> peptide like VGVAPG? Also, is there anyway to insure that all X-P bonds
>> will be in the trans isomer?
>>
>> Thanks
>> Bob Mecham
>>
>
>>From the NMR specialists I know that certain amount of cis-Pro is often
>present in synthetic peptides and may be estimated by NMR. It should be
>not such a big problem in a short peptide like one you quoted
>
>Hope this helps
>
>
>Vladimir Titov
>
>MailTo:titov@ibch.siobc.ras.ru
>http://titov.siobc.ras.ru
>
>Visit Vladimir's peptide page at:
>http://titov.siobc.ras.ru/peptide
>
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110