Date: Wed, 22 Jan 1997 09:36:24 -0600
Message-Id: <v01530506af0ce21838a0@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: Re: Recovery of lyophilized peptide
>From: anaspec@best.com
>Mime-Version: 1.0
>Date: Mon, 16 Oct 1995 21:28:43 -0600
>Old-To: swcoates@inet.net (Stephen W. Coates)
>Subject: Re: Recovery of lyophilized peptide
>Cc: Recipients of ABRF List <abrf@aecom.yu.edu>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Sender: Association of Biomolecular Resource Facilities
><abrf-request@aecom.yu.edu>
>Precedence: bulk
>
>Many short peptides (5mer or less) tend to be hygroscopic(occassionally
>some longer sequences), they do not precipitate out of the ether solution
>after cleavage from the resin. I would recommend the extraction procedure
>where the peptide is extracted from the resin using water or 10% acetic
>acid instead of the standard ether precipitation method.
>
>After the water or acetic acid extract is lyophilized, you may obtain a
>sticky solid or a white powder which melts upon exposure to air. To recover
>the sticky solit. redissolve it in the smallest volume of aqueous solution,
>transfer the solution to a 20ml scintillation vial, loosely cap the
>scintillation vial and relyophilize. After lyophilization, tighten the cap.
>Remember to obtain the tared weight of the vial. The final weight of the
>peptide can be obtained without exposing the peptide to the atmosphere.
>
>I hope this procedure is helpful.
>
>Anita Hong
>AnaSpec, Inc.
>(408)452-5055
>Fax(408)452-5059
>WWW.AnaSpec.Com
>___________________________________________________________________________
>We have a customer who is getting poor recovery of a hydroscopic peptide
>>(approximately 15 residues) upon lyophilization. Any suggestions to improve
>>recovery?
>>
>>Stephen W. Coates
>>Rockland Technologies, Inc.
>>538 First State Boulevard
>>Newport, DE 19804
>>TEL: 302-633-5898
>>TEL: 302-633-5733 (direct line)
>>FAX: 302-633-5893
>>swcoates@zorbax.com
>>http://rti.zorbax.com
>
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110